Extended Data Fig. 4: The chemical synthesis and genetic encoding of lipidation mimics.
From: Computational design and genetic incorporation of lipidation mimics in living cells
(a) The chemical synthesis of 4HexyF, 4OctyF, 4NonyF and 4DecyF. A three-step synthetic route was developed with the isolation yield of each synthetic step shown under the products. (b) The model structure of human mitochondrial PheRS in complex with 4HexyF. The gatekeeper residues colored in magenta displayed steric hindrance with 4HexyF. (c) The model structure of LipRS-1 in complex with 4HexyF (cyan). Residues around 4HexyF were shown as sticks with three key mutations highlighted in magenta and the others in blue. (d) The amber suppression assay of lipidation mimics by the indicated LipRS-2 variants (n = 2 biologically independent experiments). (e) The gate strategy of FACS used in Fig. 4a.
