Extended Data Fig. 3: Indel formation rates with CjCas9 and evoCjCas9 at target sites with different PAMs.
From: Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility
Mean indel frequency in self-targeting libraries on canonical (a) and non-canonical (b) PAM sites. Bars represent mean with error bars representing standard error of the mean, with individual data points (n) of biological triplicates shown as black dots (a) and indicated above error bars (a, b). The in vitro PAMDA assay (Fig. 2a) revealed few motifs recognized by CjCas9 that lie outside of the simplified N3VRYAC motif (for example N3MACAN), albeit with low efficiency. We define sequences that fall outside of N3VRYAC as non-canonical motifs for the purpose of simplification.
