Extended Data Fig. 1: Base editing assigns essentiality to cysteines targeted by anti-cancer drugs.
From: Assigning functionality to cysteines by base editing of cancer dependency genes
a, b, Heatmap showing the mutagenesis effects of Adenine Base Editors (ABE) (a) and Cytosine Base Editors (CBE) (b) on different amino acid residues. The total percentage is normalized to 100% per row. c, Schematic showing the design of lentivirus vectors delivering base editor libraries used in this study. CDA: cytidine deaminase; TadA: deoxyadenosine deaminase; UGI: uracil glycosylase inhibitor; PuroR: puromycin resistance gene. d, Waterfall plots for ABE (left) and CBE (right) libraries showing the dropout significance calculated per EGFR residue between day 16 vs day 1 from saturated scanning experiments using PC14 cells (cutoff: LFC < −0.5; p < 0.05). Data represent average values of two independent experiments. One-sided p values were calculated using randomization tests (no multiple comparison adjustments, see source code in Github).
