Extended Data Fig. 9: AA-14 promotes hormone secretion and regulates metabolism through activating GPR101.
From: Structure of GPR101–Gs enables identification of ligands with rejuvenating potential
a, Growth hormone secretion from GH3 cells transfected with siGpr101 or control siRNA in response to stimulation with 100 μM AA-14. Data are from three independent experiments (n = 3). b-c, Plasma growth hormone (b) and IGF-1 (c) levels in Gpr101f l/f l or Pomc-cre+/−Gpr101f l/f l mice treated with AA-14 (0.1 mg/kg/d) or control vehicle (n = 6 mice per group). d, Energy expenditure of the 8-week-old or 18-month-old mice treated with AA-14 (0.1 mg/kg/d) or control vehicle for 2 months (n = 6 mice per group). e, mRNA levels of Glb1, p21, Il6 and Il1α in the skin of mice treated with AA-14 or control vehicle for 2 months (n = 3 mice per group). For a-e, all data are presented as mean ± SEM values. P values were calculated using unpaired two-sided Student’s t-test (a) or two-way ANOVA with Dunnett’s post hoc test (b-e), and are indicated in the graphs.
