Extended Data Fig. 3: Motility of dyneinGal in the presence of unlabeled Lis1.
From: Lis1 slows force-induced detachment of cytoplasmic dynein from microtubules
a) Representative kymographs of dyneinGal with increasing concentrations of unlabeled Lis1. Assays were performed in 1 mM ATP and 150 mM KAc. b) The velocity of dyneinGal under different Lis1 concentrations (mean ± s.e.m.; N = 611, 694, 230, 260, 251, and 852 from left to right; two biological replicates). c) 1-CDF of run time and run length of dyneinGal under different Lis1 concentrations. Fitting to a double exponential decay (solid curves) reveals the weighted average of run time and run length of the motor in each condition (bar graphs, ± s.e.; N = 611, 694, and 852 from left to right). d) Representative kymographs of dyneinGal in the presence or absence of 1,000 nM unlabeled Lis15A. e) The velocity, run time, and run length of dyneinGal in the presence or absence of 1,000 nM Lis15A (N = 610 and 209 from left to right). The center line and whiskers represent the mean and s.d., respectively. In c and e, P values were calculated by a two-tailed t-test with Welch correction for velocity and by the two-tailed Kolmogorov-Smirnov test for run time and run length.
