Fig. 5: HFD Chk2−/− mice show improved GSIS and glucose homeostasis.
From: Checkpoint kinase 2 controls insulin secretion and glucose homeostasis
a,b, Dynamic GSIS (a) and AUC (b, P = 0.012) of primary mouse islets from chow-fed wild-type and Chk2−/− mice. n = 7 wild-type mice; n = 12 Chk2−/− mice. The data were normalized to baseline. c, Fed glucose levels in 8-month-old male HFD-fed wild-type and Chk2−/− mice. P = 0.00005. n = 12 wild-type mice; n = 9 Chk2−/− mice. d, Fed insulin levels of 8-month-old male HFD-fed wild-type and Chk2−/− mice. P = 0.034. n = 13 wild-type mice; n = 12 Chk2−/− mice. e, OGTT of 8-month-old male HFD-fed wild-type and Chk2−/− mice. P value: 30 min, P = 0.0138; 60 min, P = 0.036. n = 11 wild-type mice; n = 8 Chk2−/− mice. f, Insulin levels during OGTT of 8-month-old male HFD-fed wild-type and Chk2−/− mice. P value: 0 min, P = 0.026; 15 min, P = 0.0002. n = 10 wild-type mice, n = 6 Chk2−/− mice. g, Active GLP-1 levels during OGTT of 8-month-old male HFD-fed wild-type and Chk2−/− mice. n = 4 wild-type mice; n = 5 Chk2−/− mice. h, Glucose levels during OGTT of 8-month-old female HFD-fed wild-type and Chk2−/− mice. n = 5 wild-type mice; n = 3 Chk2−/− mice. i, Insulin levels during OGTT of 8-month-old female HFD-fed wild-type and Chk2−/− mice. P = 0.036. n = 15 wild-type mice; n = 8 Chk2−/− mice. Mice were fasted overnight for all GTT and GSIS experiments. Data represent the mean ± s.e.m. For b–i, P value was calculated with two-sided Student’s t-test. Statistical significance: *P < 0.05, ****P < 0.0001. NS, not significantly different, WT, wild type.
