Extended Data Fig. 6: Chemical structure and photophysical property of BODIPY.
From: Micropolarity governs the structural organization of biomolecular condensates
a, The structure of BODIPY-NHS probe and labelled BODIPY structure on the N-terminus or lysine residuals of ELP. b, Chemical structure of BODIPY-Halo. The chromophore core part of BODIPY-Halo resembles the chemical structure of labelled BODIPY, whereas the HaloTag reactive warhead does not affect the photophysical property of BODIPY. BODIPY-Halo was used for photophysical property measurements. c, Fast FLIM images of BODIPY-Halo in glycerol at different temperatures. The viscosity of glycerol is known to change dramatically in response to temperature changes, hence was used for the calibration of the BODIPY viscosity response. Scale bars, 10 µm. d, BODIPY lifetime-viscosity calibration curve calculated from measured BODIPY fluorescence lifetime in glycerol at different temperatures. e, Fluorescence lifetime decay curve of BODIPY-Halo in samples described in (c-d). f, Fluorescence lifetime decay curve of BODIPY-Halo in non-viscous solvents with different polarity. BODIPY displayed minimal fluorescence lifetime changes in response to different polarities. g, Excitation and emission spectra of BODIPY-NHS in pure methanol. h, Excitation and emission spectra of BODIPY-NHS in pure glycerol. The viscosity values of glycerol at different temperatures are available in Supplementary Table 4.
