Extended Data Fig. 10: Photophysical property of S-SBD-Halo.
From: Micropolarity governs the structural organization of biomolecular condensates
a, Fast FLIM images of S-SBD-Halo in solvent mixtures of methanol and 1,4-dioxane. Scale bars, 10 µm. b, SBD lifetime-dielectric constant calibration curve calculated from measured SBD fluorescence lifetime in methanol and 1,4-dioxane solvent mixtures with known dielectric constant. The dielectric constant values of methanol-1,4-dioxane mixtures were calculated by the weighted average of the mixture components by assuming a simple additive effect. The dielectric constant values of methanol-1,4-dioxane mixtures was available in Supplementary Table 3.
