Extended Data Fig. 1: Analyzation of the phase transition temperature and purity of ELP.
From: Micropolarity governs the structural organization of biomolecular condensates
a-f, Phase transition processes of ELP were captured by the rapid increase of absorbance signal using a UV-Vis-NIR spectrometer. ELPs were mixed in buffers (50 mM HEPES, pH 7.0) with different molarity of NaCl (0.15 M, 0.5 M, 1.0 M, 1.5 M, 2.0 M) at a final peptide concentration of 70 µM on ice. An assay was designed to gradually increase the sample temperature from 1 ˚C to 50 ˚C at 0.5 ˚C/min rate, while the absorbance at 395 nm was recorded for every 0.5˚C of temperature increment. g, Summary of the phase transition temperature of ELPs at different NaCl concentrations. h, SDS-PAGE analysis of the purity of the ELP. The polyacrylamide gel was stained in 0.5 M CuCl2 solution to develop protein bands.
