Extended Data Fig. 2: Fluorophore labelling does not alter the organization and partition of dual-component ELP condensates.
From: Micropolarity governs the structural organization of biomolecular condensates
a, Swapping fluorophores on ELP preserved the organization of dual-component ELP condensates. Repeating dual colour imaging from Fig. 1c with swapped labelling fluorophores yields the dual component ELP condensates with identical organizations but inverted colours. b-c, Dual colour confocal images with varying concentrations of labelled ELPs yield similar core-shell structures with identical partition coefficients. b, Dual-color confocal images of V5A2G3-120/V-120 condensates with 0.5% (experimental condition), 1% and 1.5% fluorescent labels. c, Selected areas and values of partition coefficient calculated from the images in (b). Data represent mean and standard deviations from 3 distinct samples. Statistical significance was calculated using two-tailed t-tests. ns, non-substantial. p = 0.75 between 0.5% and 1.0% labelled ELPs, p = 0.42 between 1.0% and 1.5% labelled ELPs and p = 0.49 between 0.5% and 1.5% labelled ELPs. Experimental condition: 70 µM V5A2G3-120 and 70 µM of V-120 labelled with different percentages of fluorescein-NHS and AF 647-NHS were mixed in phase separation buffer (50 mM HEPES, pH 7.0, 2 M NaCl) and incubated at RT. Scale bar, 10 µm.
