Extended Data Fig. 6: Conformational changes induced by guide-target binding and propagation.
From: Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system
a. The cartoon representation of helixentry withdrawal upon guide-target binding. b. D-loop flipping upon the propagation of guide-target. c. Structure superimposition of SPARTA-apo, Monomer-GT1, Monomer-GT2 and dimer shows consecutive and unidirectional movement of the D-loop. d. The conformational changes likely coupling C-tail/TIR release with guide-target propagation. The structure of Monomer-GT2 (light blue) is superimposed onto Monomer-GT1 (grey), and the regions of substantial conformational changes in Monomer-GT2 are colored in yellow, orange, blue, and magenta.
