Extended Data Fig. 7: Conformational changes of the SPARTAgRNA complex upon target ssDNA binding.
From: Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system
(a) Structural comparison between the SPARTAgRNA binary and the protomer 2 of the tetrameric SPARTAgRNA-ssDNA complex. The sensor loop and sensor helix regions are indicated. (b and d) Effect of sensor loop and sensor helix mutants together with the C-tail deletion mutant on NADase activity. Data are representative of three independent experiments. Data are presented as mean values ± s.d. (c) SDS-PAGE profiles of SPARTA mutants. ΔC-tail indicates deletion of the C-tail in the APAZ domain. KHR and KE mutants indicate mutation of residues K354, H358, R361 in the APAZ domain and K325, E327 in the PIWI domain to alanines, respectively. Loop GS linker indicates the replacement of Y321PIWI to Y328PIWI with a GS linker. A MBP tag is fused to the N terminal of TIR-APAZ subunit in all SPARTA mutants. Data are representative of three independent experiments.
