Extended Data Fig. 9: Size exclusion chromatography analysis of SPARTA mutants.
From: Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system
(a) Size exclusion chromatography (left) and SDS-PAGE profiles (right) of SPARTA mutants. E133-D137 (GSGSG) indicates the replacement of loop (133-EERVD-137) with a GSGSG linker in the MID domain. G42P and E50A indicate substitution of residues G42 and E50 in the TIR domain into proline and alanine, respectively. A MBP tag is fused to the N terminal of TIR-APAZ subunit in all SPARTA mutants. Red asterisk indicates the individual MBP tag. Data are representative of three independent experiments. (b) The left panel indicates size exclusion chromatography analysis of SPARTA E77A mutant. Then the tetrameric SPARTAE77A-gRNA-ssDNA complex was reloaded for a second round of size exclusion chromatography analysis (right panel).
