Extended Data Fig. 5: Insert loop region of AG binding pocket is most tolerable of mutation when apramycin is the substrate.
From: Mechanistic plasticity in ApmA enables aminoglycoside promiscuity for resistance
Specific activities for (a) ApmA-mediated acetylation and (b) ApmA(His135Ala)-mediated acetylation of AGs were determined in vitro with 1 M AG substrate, 100 µM acetyl-CoA. Individual data points are shown for two independent replicates. Legend: APR – apramycin, pink circles. TOB – tobramycin, purple diamonds. KAN A – kanamycin A, red hexagons. KAN B – kanamycin B, blue triangles. PAR – paromomycin, yellow squares. Individual data points are shown for two independent replicates. (c) Chemical line drawing representation of apramycin acetylation mediated by His135Ala mutant. (d) Chemical line drawing illustration of tobramycin acetylation mediated by His135Ala mutant.
