Extended Data Fig. 6: Functional characterization of engineered CgCLP with various fusion domains.
From: Accelerating the design of pili-enabled living materials using an integrative technological workflow
(a)TEM images showed that Ni-NTA-decorated AuNPs were anchored onto 6His-Spa2 fibers. (b) Confocal microscopic images showed the green fluorescence emitted from SpyTag-Spa2 cells to which SpyCatcher-EGFP protein binding partners were covalently attached via Spytag-SpyCatcher interaction pairs. (c) Confocal microscopic images show the green fluorescence emitted from SpyCatcher-Spa2 cells to which SpyTag-EGFP protein binding partners were covalently attached via Spytag-SpyCatcher interaction pairs. (d) Fluorescent images and quantification analysis of the immobilization ability of Mfp3Spep-Spa2 cells. Immobilized microspheres (left) on the substrates before (top) and after (bottom) challenge with water jetting at a constant discharge pressure of 5 psi. Quantification analysis of the relative capabilities of different cells (right) with immobilized PS microspheres on the substrate. The number of immobilized microspheres was set to 100% before subjecting them to mechanical challenge with water jetting. P values are indicated above the bars. Not significant (NS) P > 0.05, ***P < 0.001; Mfp3Spep-Spa2 strain versus Spa2 strain using two-tailed t-test, from three biologically independent samples, mean ± s.d. (e) Confocal microscopic images show the green fluorescence emitted from Venus-Spa2 cells. (f) Endo-1,4-β-glucanase activity of CcEgl-Spa2 cells. The P value of CcEgl-Spa2 strain versus Spa2 strain is P = 7.0E-10. ****P < 0.0001. Statistically significant differences were calculated by using a two-tailed t-test, from three biologically independent samples, mean ± s.d. Scale bars, 200 nm in a, 2 μm in b, c, and e, 100μm in d.
