Extended Data Fig. 1: The use of the BBSniffer software for mining biopolymer-producers of bacterial cellulose (BC), gas vesicle (GV) and bacterial microcompartment (BMC).

(a) For bacteria cellulose-producer mining, 7,109 strains were detected, and 1,767 strains are grouped in industrial microorganisms based on our internal bacterial database for classification. BBSniffer scored the distance between the mined industrial microorganisms and the reference strains of Komagataeibacter xylinus E25 using a distance-based phylogenetic tree. The top five mined industrial workhorses along with recommended information about culture conditions and a gene editing tool of CRISPRi system were provided for further engineering. (b) For gas vesicle-producer mining, 489 strains were detected, and 191 strains are industrial microorganisms. BBSniffer scored the distance between the mined industrial microorganisms and the reference strains of Halobacterium salinarum 91-R6 using a distance-based phylogenetic tree. The top five mined industrial workhorses along with recommended information about culture conditions and a gene editing tool of CRISPR-Cas system were provided for further engineering of gas vesicle. (c) For bacterial microcompartment mining, 4,241 strains were detected, and 1,381 strains are industrial microorganisms. BBSniffer scored the distance between the mined industrial microorganisms and the reference strains of pathogenic Salmonella typhimurium LT2 by a distance-based phylogenetic tree. The top five mined industrial workhorses along with recommended information about culture conditions and a gene editing tool of CRISPR-Cas system were provided for further engineering of bacterial microcompartment.