Extended Data Fig. 2: Screening 8,448 cyclic peptides against thrombin.
From: De novo development of small cyclic peptides that are orally bioavailable
a, Quantification of the 384 linear dithiol peptides using Ellman’s reagent. b, Heat map with residual thrombin activity measured for each cyclic peptide, divided into the two linkers used for cyclization and the four different generated peptide formats. Individual peptide sequences are aligned on the vertical axis, and the acids used for N-acylation are on the horizontal axis. The screen was carried out at a peptide concentration of 10 μM. Cyclic peptides forming a structurally related cluster and containing the most active hit are highlighted as Group 1. c, Thrombin inhibition of replicate reactions for the 20 best hits.
