Extended Data Fig. 2: Validation of DeKinomics’ temporal resolution in phosphoproteome.
From: DeKinomics pulse-chases kinase functions in living cells
a, The numbers of identified phosphoproteins, phosphopeptides and phosphosites in DeKinomics study of BRAF with phosphorylated residue numbers from different classes according to localization probability and distribution of phosphorylated amino acids. b, Quantitative phosphoproteomics revealed the changes of phosphosites in MAPK signaling pathway upon BRAF activation. c, The KEGG pathway enrichment analysis of phosphosites with the Euclidean distance among the smallest 10%. According to the gene ratio, the top 10 pathways were depicted. Two-sided hypergeometric test followed by Benjamini-Hochberg correlation. KEGG, Kyoto Encyclopedia of Genes and Genomes. d, Dynamics of MAPK signaling pathway phosphosites that increased upon BRAF activation were showed in detail. e, Validation of phosphorylation dynamics in MAPK signaling pathway in repetitious experiments. Error bars represent mean ± SD (n = 3 independent experiments).
