Extended Data Fig. 3: SRC-driven pTyr proteome dynamics were deeply profiled by DeKinomics.
From: DeKinomics pulse-chases kinase functions in living cells
a, Western blotting validation of rapid activation of tyrosine kinase SRC (n = 2). b, The numbers of identified pTyr proteins, peptides and phosphosites in the SRC study, with the pTyr residue number from different classes according to localization probability. c, Schematic illustration of the time-resolved pTyr proteomics upon UV irradiation as a control experiment. d, Bar graph comparing the unchanged and the significantly changed pTyr sites in UV irradiation experiment. e, Violin plot indicating the increased global pTyr intensity upon SRC activation. The counts of quantified pTyr sites (n) in each time point are indicated in brackets. Box plots show median (center line), the upper and lower quantiles (box), and the minima and maxima of the data (whiskers). f, The Pearson correlation of pTyr intensity between the biological replicates. g, GO enrichment analysis of pTyr sites from different clusters.
