Extended Data Fig. 10: Examination of effects of N-glutarylspermidines on SIRT5 in vitro.
From: Acylspermidines are conserved mitochondrial sirtuin-dependent metabolites
a, maspid#3 and maspid#4 were not SIRT5 substrates. O-glutaryl-ADP-ribose was not produced when incubating 100 µM maspid#3 or maspid#4 with 1 µM recombinant SIRT5 and 1 mM NAD+ in Tris buffer (pH 7.5) for 2 hr at 37 °C. A peptide with succinylated lysine was used as positive control. n = 2. b, N-glutarylspermidines had no inhibitory effects on SIRT5. maspid#3 to maspid#4 ratio was 1:1. Nicotinamide was used as a positive control, IC50 = 22 µM. n = 2. n, number of assays using the same commercial enzyme sample.
