Extended Data Fig. 4: The disturbance in membrane structure induced by PAF.
From: PAFAH2 suppresses synchronized ferroptosis to ameliorate acute kidney injury
(A, B) The permeability assay of POPC liposomes (2% NBD-DPPE) containing different percentages of PAF C16 with DMSO or Fer-1 added in this system. Data show mean ± s.d., n = 3 independent experiments. (C, D) The snapshots of locally lipid bilayer of 50% PAF and 50% LPC16 obtained from multi-microsecond all-atom MD simulations. (D) The atomistic structures of POPC, PAF C16 and LPC 16, which are colored in green and silver and orange, respectively. (E) The comparison of water density along the normal direction of three kinds of lipid bilayers indicated. In all water density profiles, the membrane centre is located at 0 nm of the z-axis. (F) The protrusions per lipid for three bilayers compositions. (G) The interaction between the lipid tails and the water was quantified by the number of contacts per frame of each atom with water. (H) The interaction between the lipid tails and the water was quantified by the number of contacts per frame of each lipid with water. For F-H, data are averaged from two independent MD simulation.
