Extended Data Fig. 7: PAFAH2 is involved in ischemia reperfusion induced AKI and ferroptosis.
From: PAFAH2 suppresses synchronized ferroptosis to ameliorate acute kidney injury
(A-I) C57BL/6 J mice were subjected to sham operation or bilateral kidney ischemia (30 minutes) and then reperfusion. (A, B) Relative mRNA levels of Ptgs2 and Chac1 in renal cortex. Data show mean ± S.D., n = 3 (C) Immunoblots of indicated proteins in renal cortex, Actin was used as a loading control. (D-F) C57BL/6 J mice were pre-treated with vehicle or Ferrostatin-1 (5 mg/kg) and then subjected to bilateral kidney I/R. (D, E) The Levels of BUN and serum creatinine. Data show mean ± s.d., n = 4 in each group. Unpaired two-sided Student’s t-test (F) The damage of renal cortex shown by H&E staining. The immune-histochemical staining of 4-HNE, TfR1 and COX2 in renal cortex slices were shown in sequence. Scale bar, 50 μm. (G) Immunoblots of PAFAH2 protein in renal cortex. Actin was used as a loading control. (H) The immunohistochemical staining of PAFAH2 in renal cortex slices. Scale bar, 50 μm. (I) The percentage of PAFAH2 stained tubules were quantified. Data show mean ± s.d., n = 4. Unpaired two-sided Student’s t-test.
