Extended Data Fig. 4: OligoD insertion into SARS-CoV-2 spike.
a, Size-exclusion purification and gel electrophoresis of wild-type or oligoD-modified spike. Gel was stained with Coomassie brilliant blue. b, SEC-MALS analysis of spike and spike-12D on a Superdex 200 column. UV and LS indicate absorbance at 280 nm and light scattering signals, respectively. c,d, Thermal melting profiles and Tm of wild-type and oligoD-modified spike in the absence (c) or presence (d) of alum (spike: alum = 1:10, w/w in d) (n = 3 samples per group). e, BLI binding profiles of wild-type or oligoD-modified spike with ACE2-Fc and three Spike-specific mAbs (COVA2-15, CB6 and CR3022). A GP-specific mAb (mAb114) served as a negative control. Vertical dashed lines indicate the beginning of dissociation.
