Extended Data Fig. 3: Analysis of germinal center responses.
a, Serum GP-specific IgG2a or IgG2b titers 7 d, 14 d or 21 d post-immunization (n = 10 mice per antigen per time point). Dashed lines indicate the limit of quantification. Data are presented as the geometric mean ± s.d. of the log10‐transformed values. b, Gating strategy for the analysis of germinal center B cells (CD19+CD95+CD38−), IgG+ germinal center B cells and T follicular helper cells (CD3+CD4+PD1+CXCR5+) in the draining lymph nodes. c, Analysis of IgG+ GC B cell responses after immunization. Each circle represents a single mouse (n = 10). Data are presented as mean ± s.d. Comparison of two groups was performed using the two-tailed Mann–Whitney U-test. P values of 0.05 or less were considered significant.
