Table 1 List of detected enzymes involved in lignocellulose biomass degradation with their taxonomic assignments and ROI-specific localization

From: Mapping microhabitats of lignocellulose decomposition by a microbial consortium

Substrate specificity

KO number

EC number

Enzyme

Taxonomic annotation(s)

LM1 (n = 3)

LM2 (n = 3)

LM3 (n = 6)

PMM (n = 3)

Lignin

K20929

1.2.3.15

Glyoxal/methylglyoxal oxidase

Fungi

O

O

O

X

Hemicellulose

K01181

3.2.1.8

Endo-1,4-β-xylanase

Fungi/Leucoagaricus

O

X

O

X

Hemicellulose

K01209

3.2.1.55

α-l-Arabinofuranosidase

Fungi/Leucoagaricus

O

O

O

O

Hemicellulose

K15920

3.2.1.37

Exo-1,4-β-xylosidase

Fungi/Leucoagaricus

O

O

O

X

Hemicellulose

K01192

3.2.1.25

Exo-β-d-mannanase

Fungi

X

O

O

X

Hemicellulose

K19355

3.2.1.78

Endo-1,4-β-mannanase

Fungi

X

O

X

X

Hemicellulose

K07407

3.2.1.22

α-Galactosidase

Leucoagaricus

X

O

O

X

Hemicellulose/cellulose

K05349

3.2.1.21

β-Glucosidase

Fungi/Leucoagaricus

O

O

O

X

Starch

K01178

3.2.1.3

Glucoamylase

Fungi/Leucoagaricus

O

O

O

X

Pectin

K01051

3.1.1.11

Pectinesterase

Fungi/Leucoagaricus

O

O

O

X

Pectin

K01728

4.2.2.2

Pectate lyase

Fungi/Leucoagaricus

O

O

O

O

Pectin

K18195

4.2.2.23

Rhamnogalacturonan endolyase

Fungi/Leucoagaricus

X

O

O

X

Pectin

K01213

3.2.1.67

Galacturan 1,4-α-galacturonidase

Fungi

X

O

O

X

Pectin

K15530

3.1.1.86

Rhamnogalacturonan acetylesterase

Fungi/Leucoagaricus

O

O

O

X

Pectin

K18106

1.1.1.-

d-Galacturonate reductase

Fungi/Leucoagaricus

O

O

O

X

Cellulose

K01179

3.2.1.4

Endoglucanase

Fungi/Leucoagaricus

O

O

O

X

Cellulose

K01225

3.2.1.91

Cellulose 1,4-β-cellobiosidase

Fungi/Leucoagaricus

O

O

O

X

Chitin

K01183

3.2.1.14

Chitinase

Fungi/Arthropodaa

O

O

O

O

  1. A full list of peptides assigned to the cluster and corresponding KO numbers along with their functional annotation, taxonomic assignments and ROI-specific localization have been included in Supplementary Table 5. A peptide was considered present if it was detected in at least two section replicates within each ROI. The cluster was identified with at least two peptides detected within the ROI that previously met peptide identification criteria.
  2. O, detected; X, absent or below detection limits.
  3. aThe chitinase related to the phylum Arthropoda was localized in the PMM ROI.