Table 1 Identification of candidate genes for downstream pathway steps

From: Complete biosynthesis of the potent vaccine adjuvant QS-21

Gene ID

Enzyme class

Annotation

Score

Qs0315350

Characterized gene

Terpene cyclase/mutase family member (QsbAS1)

3.00

Qs0322000

Characterized gene

CYP (CYP716A297)

3.00

Qs0321930

Characterized gene

Glycosyltransferase (UGT74BX1)

3.00

Qs0259300

Characterized gene

CYP (CYP716A224)

3.00

Qs0321920

Characterized gene

Glycosyltransferase (UGT91AR1)

3.00

Qs0123860

Characterized gene

Glycosyltransferase (UGT73CU3)

3.00

Qs0268880

PKSIII

Chalcone synthase (PKS5)

3.00

Qs0283870

Characterized gene

Glycosyltransferase (UGT73CX1)

3.00

Qs0321940

Characterized gene

Glycosyltransferase (UGT91AP1)

3.00

Qs0322030

Acyltransferase

Vinorine synthase-like (ACT2)

3.00

Qs0234120

Characterized gene

Glycosyltransferase (UGT91AQ1)

3.00

Qs0283850

Characterized gene

Glycosyltransferase (UGT73CX2)

3.00

Qs0234150

Glycosyltransferase

Glycosyltransferase

2.91

Qs0234050

Other

CYP

2.80

Qs0326850

Reductase

Cinnamoyl-CoA reductase (KR1)

2.50

Qs0175050

Other

ATP-citrate synthase β-chain protein

2.50

Qs0162400

Other

Squalene monooxygenase-like

2.50

Qs0081220

Other

CYP

2.50

Qs0264740

Acyltransferase

Anthocyanin 5-aromatic acyltransferase (ACT3)

2.50

Qs0149710

Other

ATP-citrate synthase α-chain protein

2.50

Qs0131010

Glycosyltransferase

Glycosyltransferase (UGT73CZ2)

2.50

Qs0233700

Glycosyltransferase

Glycosyltransferase

2.50

Qs0082410

Glycosyltransferase

Glycosyltransferase

2.50

Qs0152180

Glycosyltransferase

Glycosyltransferase

2.50

Qs0098630

Acyltransferase

Vinorine synthase-like

2.50

Qs0298840

Other

Trifunctional RHM1-like

2.50

Qs0007520

Other

2-Oxoglutarate and Fe(II)-dependent oxygenase superfamily

2.50

Qs0264720

Acyltransferase

Malonyl-coenzyme:anthocyanin 5-O-glucoside-6′′′-O-malonyltransferase

2.50

Qs0124580

Other

ATP-citrate synthase α-chain protein

2.50

Qs0020840

Other

Patellin-3-like

2.50

Qs0287320

Other

4-Coumarate-CoA ligase-like

2.50

Qs0004900

Other

ATP-citrate synthase β-chain protein

2.50

Qs0264710

Acyltransferase

Malonyl-coenzyme:anthocyanin 5-O-glucoside-6′′′-O-malonyltransferase

2.50

Qs0098610

Glycosyltransferase

Glycosyltransferase

2.50

Qs0285490

PKSIII

Chalcone synthase (PKS6)

2.50

Qs0235370

Reductase

Very-long-chain 3-oxoacyl-CoA reductase 1 (KR2)

2.50

Qs0060960

Other

Tubulin β-chain

2.45

Qs0091410

Other

C2 domain-containing protein

2.43

Qs0234130

Characterized gene

Glycosyltransferase (UGT73CY3)

2.38

Qs0283860

Glycosyltransferase (partial)

Glycosyltransferase (partial)

2.30

  1. The top 40 Q. saponaria candidate genes for QS-21 biosynthesis. The overall scores were determined by the strength of gene coexpression with QsbAS1, absolute transcript abundance in primordial tissue and presence in putative BGCs determined by a modified version of plantiSMASH28 (see Methods for details). Previously characterized QS-21 pathway genes7 are indicated in bold. PKS5 and PKS6 (annotated as PKSIIIs) are also in the top 40. Candidates for the missing steps for addition of the acyl chain are listed with their corresponding enzyme class, whereas genes irrelevant to this work are labeled as ‘Other’.