Fig. 2: Maturation of FUS NTD liquid droplets followed by solid-state NMR in real time.
From: A solid beta-sheet structure is formed at the surface of FUS droplets during aging
a, Time-dependent 1H–13C CP-MAS and 1H–13C INEPT spectra of biphasic FUS (left) and time-dependent intensity changes during 2 days of maturation for several selected resonances (right; the spectral regions used are highlighted by colored rectangles in the spectra). The intensity of the CP spectrum recorded after 2 days was normalized to 1. A linear regression is shown (straight lines) with slopes (units of 1/days) of 0.398 (carbonyl carbons, C*), 0.290 (Caromatic), 0.254 (Cα) and 0.264 (Cαglycine) for the CP spectra, and −0.062 (Caromatic), −0.077 (Cβ), −0.058 (Cα) and −0.074 (Cαglycine) for the INEPT spectra. b, Time-dependent 1H–13C CP-MAS and 1H–13C INEPT spectra of monophasic FUS (left) and time-dependent intensity changes during 2 days of maturation for several selected resonances (right; the spectral regions used are highlighted by colored rectangles in the spectra). The intensity of the CP spectrum recorded after 2 days was normalized to 1. A linear regression is shown (straight lines) with slopes (units of 1/days) of 0.283 (C*), 0.144 (Caromatic), 0.142 (Cα) and 0.120 (Cαglycine) for the CP spectra, and −0.016 (Caromatic), −0.019 (Cβ), −0.006 (Cα) and −0.003 (Cαglycine) for the INEPT spectra. All normalized intensities data are presented as signal-to-noise values ± s.d.
