Extended Data Fig. 2: Solvent behavior during maturation.

(a) Schematic representation of the model of FUS aggregation in coexisting phases. (b,c) Time evolution of aggregate and solvent volume fractions in the droplet (I) and dilute (II) phases for the case when (b) monomer-solvent interactions are disfavored over aggregate⁻solvent interactions (\({\chi }_{1s} > {\chi }_{2s}\)), or (c) aggregate-solvent interactions are disfavored over monomer-solvent interactions (\({\chi }_{1s} < {\chi }_{2s}\)). See Methods for parameters used in the plots. (d) Solvent volume fraction as a function of the total aggregate concentration formed during the reaction \(\bar{{\phi }_{2}}\) for two different initial conditions, \({\phi }_{{tot}}=0.55\) (solid lines) and \({\phi }_{{tot}}=0.8\) (dashed lines). Simulations are performed in the regime \({\chi }_{1s} > {\chi }_{2s}\) using the parameters in panel (b).