Extended Data Fig. 8: AT118-L AT1R Data Processing.
From: Antibodies expand the scope of angiotensin receptor pharmacology
a) Size exclusion trace, b) SDS-PAGE gel under reducing conditions, and c) cryo-EM data processing scheme of AT118-L AT1R-BRIL, anti-BRIL Fab, anti-Fab nanobody complex. One purification of this complex was performed and similar size-exclusion and SDS-PAGE results are in agreement with the analogous complex prepared in Extended Data Fig. 4. d) Fourier shell correlation (FSC) used to determine the global map resolution. e) FSC used to determine locally refined map resolution. f) Experimental density of losartan within the orthosteric binding pocket from locally refined map. g) Comparison of orthosteric binding pocket in AT118-L, Losartan, AT1R-BRIL complex (blue) and olmesartan bound AT1R (purple, PDB 4ZUD).
