Extended Data Fig. 5: MMH2-Biotin characterization suggests limited off-target activity of covalent degraders.
From: Template-assisted covalent modification underlies activity of covalent molecular glues
a. Chemical structure of MMH2-Biotin. b. Biotin pull-down followed by mass spectrometry in K562 cell lysates treated with either MMH2-Biotin at 1 μM (n = 3), or DMSO at 1 μM only (n = 3). Fold enrichment and p-values were calculated by comparing MMH2-Biotin treated samples to DMSO control samples. Statistical analysis was performed using a two-sided moderated t-test as implemented in the limma package. c. Flow cytometry analysis of BRD4BD2-eGFP degradation in K562 cells that were treated with increasing concentrations of MMH2 or MMH2-Biotin for 16 h (n = 3). d. TR-FRET signal for DDB1-DCAF16-BODIPY to BRD4BD2-terbium with increasing concentrations of MMH2 or MMH2-Biotin (n = 3).
