Extended Data Fig. 5: Comparison of editing efficiencies among IminiCBE, SIminiCBE, enIscBn-CBE and SpGn-CBE.
From: Engineering miniature IscB nickase for robust base editing with broad targeting range
a, Diagram of IminiCBE, SIminiCBE, enIscBn-CBE and SpGn-CBE. EnIscBn is an IscB nickase variant containing D61A, E85R, H369R, S387R and S457R mutations. IscBnQM is an IscB nickase variant containing D61A, D97K, F138N, S431K and S457R mutations. SpGn is an SpCas9 nickase variant containing D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q and T1337R mutations. b, Diagram of the TAM/PAM matched sites for IminiCBE, SIminiCBE, enIscBn-CBE and SpGn-CBE. c, Comparison of C-to-T editing efficiencies among IminiCBE, SIminiCBE, enIscBn-CBE and SpGn-CBE at 9 target sites in HEK293FT cells. All data are means ± s.d. (n = 3 independent experiments).
