Extended Data Fig. 6: Assessment of the enrichment capabilities of the three antibodies.
From: Lysine l-lactylation is the dominant lactylation isomer induced by glycolysis
Related to Fig. 4. a) An illustration depicting the experimental setup for assessing antibody enrichment. In each immunoprecipitation (IP) experiment, a trace amount (0.3 ng) of three synthetic peptides (GGK*GLGK, QLATK*AAR, and PELAK*SAPAPK) were mixed with a larger quantity of recombinant histone peptides (100 µg). The standard IP-LC-MS/MS procedure was followed, and the peptide intensities were measured to calculate the ratio of intensities ‘after IP’ compared to ‘before IP’. Panel b) displays the resulting intensity ratios for the three modified peptides, which indicate the relative enrichment performance of the antibodies.
