Extended Data Fig. 6: The chemical characterization of SMART1.
From: Targeting Smurf1 to block PDK1–Akt signaling in KRAS-mutated colorectal cancer
a, Structure–activity relationship of the tricyclic system. IB analysis of Smurf1 and Smurf2 in HCT116 cells treated with indicated compounds (1 μM, 12 h). b,c, Representative images of SPR binding assay of SMART1d (b) or SMART1e (c) to full-length Smurf1. d, Dose–response curves for HCT116 cells treated with SMART1, SMART1d or SMART1e for 72 h (data are presented as mean ± s.e.m. of 4 technical repeats from one of three independent experiments). Immunoblots are representative of three independent experiments.
