Fig. 4: High-throughput screening of DHDs.
From: Massively parallel measurement of protein–protein interactions by sequencing using MP3-seq
a, Summary of the synthetic binder pair types. b, Successful designs for the three main sets. Gray bars are possible pairs for the set and colored bars indicate the number of successes. c, Top five successful designs from each set by P-LFC. Yellow boxes show on-target interactions. The DHD1 and DHD0 subset had three biological replicates, the DHD0, DHD2 and mALb subset had one biological replicate and the full set had two replicates. Biological replicates for overlapping all-by-all PPI measurements were combined using the data pipeline. d, From left to right, pre-DUET positive P-LFC Padj ≤ 0.01 PPI network for the DHD1 and DHD0 subset and the network at DUET iterations 50, 75 and 113 (final). e, Pre-DUET network for the DHD0, DHD2 and mALb sets and the network at DUET iterations 10, 15 and 37 (final). f, Pre-DUET network for all designs and the network at DUET iterations 50, 75 and 114 (final). g, Orthogonality gaps of the DUET final networks without significance filtering. Left and right dashed lines show the MP3-seq orthogonality gaps for Bcl-2 and final inhibitors and the NCIP series, respectively. Yellow squares correspond to half of the starting networks remaining. h, DUET networks reduced to half their final iteration size.
