Extended Data Fig. 5: RNA-splicing only logic gates.
From: Programmable trans-splicing riboregulators for complex cellular logic computation
a-c, Fluorescence for the two-input AND gates from ECF20 (a), sfGFP (b), and mCherry (c). d-e, Fluorescence for the two-input NAND gates from LmrA, PhlF (d), and BM3R1 (e). f, Fluorescence for the two-input NIMPLY gates. g, Fluorescence for the three-input NAND gate from BM3R1. h, Fluorescence for the four-input AND gate. Inset, fluorescence levels on logarithmic scales. Data in (a-b, d-h) were collected by flow cytometry. Data in (c) were collected by plate reader. Bars and errors represent the mean values and s.d. of six biological repeats (n = 6) in (g) and of three biological repeats (n = 3) in other panels. Bacteria transformed with empty vectors and J23101-sfGFP/mCherry were used as negative and positive controls for calculating fluorescence values in relative promoter units (RPU). The fold changes of logic gates were calculated by dividing the lowest TRUE-state fluorescence values by the highest FALSE-state fluorescence values, and labeled above the bars.
