Extended Data Fig. 6: ROS-related genetic or chemical perturbations did not affect ROS accumulation, cell death or biofilm expansion rate in normal medium.
From: Community-specific cell death sustains bacterial expansion under phosphorus starvation
a-c, Average DCFH-DA fluorescence (n = 3), PI fluorescence (n = 3), and biofilm expansion rates (n = 6 for wild type biofilms, n = 3 for mutant strains and +DMSO group) before phosphate removal. All data points were biological replicates. d-f, Average DCFH-DA fluorescence, PI fluorescence, and biofilm expansion rate 24 h after 2-AEP removal. n = 2 biological replicates. g-i, Average DCFH-DA fluorescence, PI fluorescence, and biofilm expansion rate before 2-AEP removal. n = 2 biological replicates. Error bars indicate mean values ± SD. Statistical significance in relevant panels was determined by two-sided Student’s t-test; ns, not significant, P ≥ 0.05.
