Extended Data Fig. 8: Counting percentage of cell death after phosphorus removal.
From: Community-specific cell death sustains bacterial expansion under phosphorus starvation
Bacteria from biofilm or planktonic culture were stained with the fluorescent death marker PI, imaged by fluorescence microscopy. The images are shown under the same contrast setting; representative of 3 biological replicates. Reference dead bacteria (the image on the right) were obtained by killing the bacteria in 80 °C water bath for 20 min. The percentage of cells with high PI fluorescence was calculated at various time points after phosphorus removal. For each condition and time point, > 300 bacteria were counted. Scale bar, 10 μm.
