Extended Data Fig. 4: A covalent fluorescent light-up aptamer (coFLAP) examplified for Pepper.
From: Engineering covalent small molecule–RNA complexes in living cells
a, Relative conversion–time plot at different pH for the reaction between MsOc3HBC and Pepper RNA (conditions: 2.5 μM RNA, 50 μM with MsOc3DPQ1 or Brc3DPQ1, 150 mM KCl, 2.0 mM MgCl2, 50 mM buffer, 37 °C, pH values as indicated). Individual data points are shown as open circles. Mean values (filled circles) are reported ± s.e.m. Measurements were performed in triplicate. b, Comparison of fluorescence emission spectra of Pepper with non-covalently bound HBC (conditions: 0.5 µM RNA, 0.5 µM HBC, 100 mM KCl, 2.0 mM MgCl2, 50 mM MES buffer, pH 6.0) and c3HBC modified Pepper (conditions: 0.5 µM RNA-c3HBC, buffer conditions as above).
