Extended Data Fig. 7: RNA-seq analysis of HEK293T cells after Pepper ligand treatment.
From: Engineering covalent small molecule–RNA complexes in living cells
Cells were incubated with 10 µM HBC530, MsOc3HBC or DMSO (control) for 5 h before RNA extraction and RNA-seq. a, Differential gene expression analysis indicates dysregulation (log2 fold change ≥ 1, padj ≤ 0.05; two-sided Wald test with Benjamini-Hochberg correction for multiple testing) of only five genes in HBC530 and 11 genes in MsOc3HBC-treated cells. b, Integrative Genomics Viewer (IGV) tracks of four example genes do not show premature termination of reverse transcription.
