Extended Data Fig. 2: Reactivity scope of Tt preQ1 aptamer tethering.
From: Engineering covalent small molecule–RNA complexes in living cells
a, Relative conversion–time plot for the different ligands vs wt and C15U Tt RNAs. Individual data points (open circles) (n = 3 independent experiments), mean ± s.e.m. (black circles); conditions: 2.5 μM RNA, 125 μM ligand, 100 mM KCl, 2.0 mM MgCl2, 50 mM MES buffer pH 6.0, 37 °C). b, The reaction rate is dependent on ligand concentration (depicted for Brc3preQ1 vs wt Tt RNA). Relative conversion vs time (top plot) and initial reaction rate vs ligand concentration (bottom plot). The observed rate constants kobs were determined based on HPLC trace analysis at four concentrations of Brc3preQ1, ranging from 85 to 435 μM (conditions: 17 nM RNA, 100 mM KCl, 2.0 mM MgCl2, 50 mM MES buffer pH 6.0, 37 °C. The red line represents a curve fit to kobs = kmax[Brc3preQ1]/(KM,app + [Brc3preQ1]). Individual data points (open circles) (n = 3 independent experiments), mean ± s.e.m. (black circles). c, Same as b but for Brc3DPQ1 vs C15U Tt RNA; Brc3DPQ1 ranging from 125 to 625 μM (conditions: 25 nM RNA, 100 mM KCl, 2.0 mM MgCl2, 50 mM MES buffer pH 6.0, 37 °C.
