Extended Data Fig. 1: Gating Strategy for FACS and flow cytometry analysis of K562s and T cell expansion.
From: Orthogonalized human protease control of secreted signals
a, Gating strategy used to sort K562s engineered to express IL-2 hDIRECT (mCherry+) from lentiviral transduced population used in the T cell expansion assay on Fig. 6b-c. b, Gating strategy to analyze T cell expansion with co-incubation with hDIRECT-engineered K562s (mCherry+). Cells and singlets were liberally gated to include all possible cells and to identify T cells (mCherry-) for analysis in Fig. 6b.
