Extended Data Fig. 7: LC-MS identification of CoxGNT associated molecules.
From: Quinone extraction drives atmospheric carbon monoxide oxidation in bacteria
(a) High-performance liquid chromatography-mass spectrometry (LC–MS) analysis of the Folch extract from purified CoxGNT. The base peak chromatogram (left) shows a peak at 21.65 min, corresponding to an ion at m/z = 746.609 (b), which is consistent with the ammonium adduct of ubiquinone-8, m/z = 746.608. (c) The composite omit map of electron density within the hydrophobic cavity of the crystal structure of CoxGNT contoured at 1 σ, fitted with ubiquinone-8. (d) A subset of the different docking poses occupied by MQ9 in AutoDock Vina docking into CoxGNT. (e) High-performance liquid chromatography-mass spectrometry (LC-MS) analysis of Folch extracts from CoxGNT, with a substantial peak at 23.95 min in the menaquinone-9 incubated sample. (f) The mass spectrum of the species eluting at 23.95 min, which has a m/z = 802.649, consistent with the ammonium adduct of menaquinone-9, m/z = 802.665.
