Extended Data Fig. 1: Different death-inducing effect of the stereoisomers NC1 and NC1i.
From: Persistent activation of TRPM4 triggers necrotic cell death characterized by sodium overload
a, Chemical structure of compound NC1 and NC1i. b, IC50 of NC1 and NC1i in various cell lines treated for 48 h. c, Live-cell images of MCF7 cells treated with 50 nM NC1 in medium containing SytoxGreen. Scale bars, 25 μm. d, Heatmap of viability of cells pretreated for 1 h with 20 μM Z-VAD-FMK (Z-VAD), 10 μM Necrostatin-1 (Nec-1), 50 μM Chloroquine (CQ), 50 μM 3-Methyladenine (3-MA), 1 μM Ferrostatin-1 (Fer-1), 1 μM Liproxstatin-1 (Liprox-1), or 100 μM Deferoxamine (DFO) and then treated with either 100 nM NC1, RSL3, 50 ng/ml TNF-α + 1 μM Smac Mimetic (TS), or TS + 20 μM Z-VAD (TSZ) for 24 h. Viability data represent mean of n = 3 replicates from one representative of three independent experiments. Micrograph data show one representative out of three replicates from three independent experiments.
