Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Ubiquitin tools

Tracking E2-specific substrates

Advanced E2-modified ubiquitin probes enable investigation of E2-selective ubiquitination in cells and the discovery of tyrosine ubiquitination as a modification occurring in a UBE2D3-specific manner.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Tracking E2 selective ubiquitination.

References

  1. Peng, J. et al. Nat. Biotechnol. 21, 921–926 (2003).

    Article  CAS  PubMed  Google Scholar 

  2. Vere, G., Kealy, R., Kessler, B. M. & Pinto-Fernandez, A. Biomolecules 10, 1453 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Sun, M. & Zhang, X. Cell Biosci. 12, 126 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Bakos, G. et al. Nat. Commun. 9, 4776 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Sheng, Y. et al. Mol. Cell. Proteomics 11, 329–341 (2012).

    Article  PubMed  PubMed Central  Google Scholar 

  6. Shen, S. & Yin, H. Nat. Chem. Biol. https://doi.org/10.1038/s41589-024-01809-9 (2025).

    Article  PubMed  Google Scholar 

  7. Dikic, I. & Schulman, B. A. Nat. Rev. Mol. Cell Biol. 24, 273–287 (2023).

    Article  CAS  PubMed  Google Scholar 

  8. Squair, D. R. & Virdee, S. Nat. Chem. Biol. 18, 802–811 (2022).

    Article  CAS  PubMed  Google Scholar 

  9. Abdul Rehman, S. A. et al. Sci. Adv. 10, eadh0123 (2024).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Hunter, T. Curr. Opin. Cell Biol. 21, 140–146 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Benedikt M. Kessler.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jones, H.B.L., Damianou, A. & Kessler, B.M. Tracking E2-specific substrates. Nat Chem Biol 21, 976–977 (2025). https://doi.org/10.1038/s41589-025-01848-w

Download citation

  • Published:

  • Version of record:

  • Issue date:

  • DOI: https://doi.org/10.1038/s41589-025-01848-w

Search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research