Extended Data Fig. 5: Biochemical validation of dopamination in Tau40.
From: Quantitative chemoproteomics reveals dopamine’s protective modification of Tau
a, Scheme of validating dopamination of hTau by DAyne using in-gel fluorescence imaging. b, Single or double cysteine mutants abolished DAyne’s labeling of Tau40. c, DAyne’s labeling of Tau40 can be competed by native DA. For b and c, fluorescence and Coomassie brilliant blue signals were collected in parallel from the same gel. Similar results were obtained in three independent biological replicates.
