Extended Data Fig. 9: smFRET traces for the mGluR2 LBD closure reporter at 50 fps. | Nature Chemical Biology

Extended Data Fig. 9: smFRET traces for the mGluR2 LBD closure reporter at 50 fps.

From: Domain coupling in activation of a family C GPCR

Extended Data Fig. 9

a–c, mGluR2 adopts longer-lived low-FRET and high-FRET states in mGluR2/3 (a) and mGluR2/4 heterodimers (b) than in the mGluR2/7 heterodimer (c). With a Group III–specific agonist (d), mGluR2 in mGluR2/7 heterodimers occasionally populates high-FRET states. Two biological replicates (data presented as mean ± s.e.m.; 4–5 movies per histogram) show effects of LSP4-2022 on mGluR2 closure in mGluR2/7 heterodimers.

Source data

Back to article page