We developed a ligand-responsive solid-state condensate platform for on-demand spatiotemporal control of gene expression in mammalian cells. In particular, the modular design of the condensates enabled spatiotemporal capture and manipulation of DNA, RNA and protein in engineered nuclear condensate structures as an efficient alternative for regulating gene expression.
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References
Guo, H. et al. Spatial engineering of E. coli with addressable phase-separated RNAs. Cell 185, 3823–3837.e23 (2022). This paper reports synthetic membraneless organelles in E. coli for advanced bioengineering.
Dai, Y., You, L. & Chilkoti, A. Engineering synthetic biomolecular condensates. Nat. Rev. Bioeng. 1, 466–480 (2023). A review article that presents progresses and application potential of synthetic condensates.
Nie, J. et al. A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells. Nat. Commun. 16, 2449 (2025). This paper reports that LplA as a globular protein can form orthogonal condensates in eukaryotic cell.
Yoshikawa, M., Yoshii, T., Ikuta, M. & Tsukiji, S. Synthetic protein condensates that inducibly recruit and release protein activity in living cells. J. Am. Chem. Soc. 143, 6434–6446 (2021). This paper reports that the activity of proteins can by blocked by solid-like condensates.
Foight, G. W. et al. Multi-input chemical control of protein dimerization for programming graded cellular responses. Nat. Biotechnol. 37, 1209–1216 (2019). This paper reports multi-input chemical control of a switch protein NS3a for diverse outputs.
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This is a summary of: Wang, Y. et al. Programmable solid-state condensates for spatiotemporal control of mammalian gene expression. Nat. Chem. Biol. https://doi.org/10.1038/s41589-025-01860-0 (2025).
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Chemically programmable condensates for gene regulation. Nat Chem Biol 21, 1315–1316 (2025). https://doi.org/10.1038/s41589-025-01912-5
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DOI: https://doi.org/10.1038/s41589-025-01912-5
