Extended Data Fig. 2: Construction of multigene vectors and their effects on daidzein and genistein production.
From: Glyceollin biosynthesis in a plant chassis engineered for isoflavone production
a, Processes for multigene vector construction. b-c, Effects of one single vector containing all bottleneck genes for daidzein (b) or genistein (c) biosynthesis versus combinations of individual genes or vectors. GP13, AtPAL2 + MGV2 + GmCHI1B1 + GmIFS1 + GmHID + AtMYB60. GP14, AtPAL2 + GmCHS8 + GmIFS1 + GmMYB12B2 + AtMYB60. All Data represent the mean of n = 3 biologically independent samples and error bars show standard deviation. Statistical analysis was performed by using two-way ANOVA. d, Fold changes of 3 and 4 at different engineering stages.
