We developed a programmable RNA acetylation system by fusing dCas13 with an engineered NAT10 variant, enabling robust and specific installation of N4-acetylcytidine (ac4C) on target RNAs in cultured cells and live animals. This system facilitated functional studies of RNA acetylation and revealed ac4C has a distinct role in regulating transcript subcellular localization.
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References
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This is a summary of: Yu, J. et al. Programmable RNA acetylation with CRISPR–Cas13. Nat. Chem. Biol. https://doi.org/10.1038/s41589-025-01922-3 (2025).
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Targeted RNA acetylation via a dCas13-guided engineered acetyl-transferase. Nat Chem Biol (2025). https://doi.org/10.1038/s41589-025-01923-2
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DOI: https://doi.org/10.1038/s41589-025-01923-2
