Extended Data Fig. 4: FRTACs promote the membrane protein degradation in cancer cells.
From: Polyvalent folate receptor-targeting chimeras for degradation of membrane proteins
a, Western blot analysis of total EGFR levels in SKOV3 cells after treatment with 10 nM FR-Ctx (FAR 15) for the indicated time points. Quantifications of EGFR to Tubulin were shown on the right. b, Western blot analysis of total PD-L1 levels in H292 cells after treatment with 10 nM FR-Atz (FAR 15) for the indicated time points. Quantifications of PD-L1 to GAPDH were shown on the right. c,d, Western blot analysis of total TROP2 levels in SKOV3 cells after 48-h treatment with indicated FR-Stz (FAR 20, c) or treatment with 10 nM FR-Stz (FAR 20) for the indicated time points (d). Quantifications of TROP2 to Vinculin were shown on the bottom or right. e,f, HER2 levels in SKOV3 cells were examined by western blot after 48-h treatment with indicated FR-Ttz (FAR 15, e) or with 10 nM FR-Ttz (FAR 15) for the indicated time points(f). Quantifications of HER2 to Tubulin were shown on the bottom or right. g, Flow cytometry detecting the degradation of cell surface PD-L1 in HCC827 cells treated with or indicated concentrations of Atz or FR-Atz (FAR 15) for 48 h. The statistic of MFI of PD-L1 was shown on the right. Data in a-g represent three independent experiments, and shown as mean ± s.e.m.
